These three plasmids are used to transduce 293T cells and the supernatant assayed for p24 gag activity. From these original experiments, new plasmids have been devised which have more versatile ...

Researchers discover how Zika virus and related viruses replicate in human cells, illuminating potential new vaccines.

We infected human cells (293T or SupT1) with high-titer stocks of HIV-based vectors, then grew them for 30 days to insure that all the extrachromsomal forms of viral DNA were lost. We then ...

was recombinantly expressed in human embryonic kidney 293T (HEK293T) cells. A high-affinity nanobody-legobody scaffold was also employed to stabilize apoB100 and facilitate particle alignment ...