此外，团队成员也在其他V型CRISPR-Cas系统成员，如Cas12b、Cas12i和Cas12j，以及II型CRISPR-Cas9中观察到了这一现象。与Cas12a稍不同，Cas9仅在单链靶标DNA存在的情况下才会启动对pre-crRNA的spacer区的反式切割，且Cas9的HNH和RuvC结构域对于pre-crRNA的反式切割都是必需的。

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

System Biosciences (SBI) develops innovative research tools for MicroRNA research, Exosome research, and Genome Engineering tools, including the CRISPR-Cas9 SmartNuclease system and PrecisionX HR ...

The versatility of Exosome-based delivery systems offers the potential to significantly enhance the therapeutic efficacy of RNA treatments and enable the precise delivery of CRISPR/Cas9 components ...

When the CRISPR Cas9 protein is added to a cell along with a piece of guide RNA, the Cas9 protein hooks up with the guide RNA and then moves along the strands of DNA until it finds and binds to a ...

More information: Jihoon Han et al, Engineered exosomes with a photoinducible protein delivery system enable CRISPR-Cas–based ...

This poster describes the use of CRISPR-ready iPSCs, which are engineered to constitutively express Cas9, enabling the rapid generation of high-efficiency gene knockouts and CRISPR screens in a ...