The 'restriction point' in the mammalian cell cycle is another irreversible transition (G0–G1) that can be understood in the same way 30. In this case, the spring is the activity of the ...

The stain can be applied to live, detergent-permeabilized or fixed cells for quantification of their distribution among the three main phases of the cell cycle (G 0 /G 1, S and G 2 /M).