When the CRISPR Cas9 protein is added to a cell along with a piece of guide RNA, the Cas9 protein hooks up with the guide RNA and then moves along the strands of DNA until it finds and binds to a ...

Both Cas9 and Cas12a cleave DNA, but they differ in how they cut the molecule.

可识别并切割非完全配对的双链DNA、十字形结构DNA、 Holliday交叉DNA、异源双链DNA，或缓慢地切割带有切刻的双链DNA；可有效识别大于1个碱基的基因错配，广泛应用于由CRISPR-Cas9、锌指核酸酶(ZFNs ...

With vasa-Cas9, high efficiency and self-limiting suppression was achieved. Applying the CRISPR-Cas9 system to edit high GC content genomes has been challenging due to its high off-target activity.

这组作者使用基因编辑工具CRISPR-Cas9，让NANOS2基因失去活性 ... 这组作者使用精原干细胞移植（SSCT）实现了睾丸结构正常的小鼠、猪和山羊产生 ...

实验结果显示，target DNA确实可以在体外激活Cas12a的RuvC结构域来加工pre-crRNA的spacer区域。此外，团队成员也在其他V型CRISPR-Cas系统成员，如Cas12b、Cas12i和Cas12j，以及II型CRISPR-Cas9中观察到了这一现象。

Get Instant Summarized Text (Gist) CRISPR nucleases, Cas9 and Cas12, have been engineered to evade immune detection, addressing a key challenge in CRISPR-based therapies. By identifying and ...

It's clear though that these companies need time. Even with the only approved CAS9 based treatment out there, CRISPR is still years away from achieving its potential. In a recent update to ...

CRISPR Therapeutics AG is a gene editing company, which engages in the development of transformative gene-based medicines for serious diseases using its proprietary CRISPR/Cas9 platform.

CRISPR stocks are high risk but hold potential for aggressive investors. CRISPR Therapeutics and its partner Vertex Pharmaceuticals have the only approved CRISPR therapy. Several other biotech ...