which gave them better control of their experiments. Avery and his colleagues provided further confirmation for this hypothesis by chemically isolating DNA from the cell extract and showing that ...

Purification of DNA from cell extract In addition to DNA the cell extract will contain significant ... During this 2 hour incubation, you may proceed with the plasmid isolation portion of the lab.

The success or failure of 5′ RACE is determined here at the beginning of the experiment. If the reverse transcription step works efficiently, the chance of isolating clones that contain the 5 ...