Thus, the overall efficiency of RT– and real-time PCR in detecting the housekeeping gene in a limited number of picked cells was 82% (243/297 samples). Negative samples may be mostly caused by ...
So the question remains "What are the essential prerequisites for establishing a quantitative RT-PCR assay?" To date, a great deal of effort has been spent defining the "correct" conditions that ...
RNAConnect, developer of enzymatic tools for visualizing and manipulating RNA, today announced the release of the UltraMarathonRT® Two-Step RT-qPCR Kit, a next-generation tool for RNA researchers ...