In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.
So the question remains "What are the essential prerequisites for establishing a quantitative RT-PCR assay?" To date, a great deal of effort has been spent defining the "correct" conditions that ...
Thus, the overall efficiency of RT– and real-time PCR in detecting the housekeeping gene in a limited number of picked cells was 82% (243/297 samples). Negative samples may be mostly caused by ...
Quantitative polymerase chain reaction (qPCR) is a molecular technique that has reshaped the landscape of biological research and medicine due to its high sensitivity and specificity. qPCR, also known ...
or real-time RT–PCR. Fast and accurate, real-time RT–PCR has been critical in detecting, tracking and studying COVID-19. But what is it, and how does it work? Let us explain.
A GlobalData report revealed that the country’s automated quantitative PCR (qPCR ... is the introduction of the SmartChip ND ...