Thus, the overall efficiency of RT– and real-time PCR in detecting the housekeeping gene in a limited number of picked cells was 82% (243/297 samples). Negative samples may be mostly caused by ...

So the question remains "What are the essential prerequisites for establishing a quantitative RT-PCR assay?" To date, a great deal of effort has been spent defining the "correct" conditions that ...

In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.

View Full Profile. Learn about our Editorial Policies. Quantitative polymerase chain reaction (qPCR) is a molecular technique that has reshaped the landscape of biological research and medicine due to ...

A GlobalData report revealed that the country’s automated quantitative PCR (qPCR ... is the introduction of the SmartChip ND ...

The UltraMarathonRT ® RT-qPCR Kit dramatically improves data reliability in quantitative PCR by maintaining consistent cycle threshold (Ct) values, regardless of RT primer type, RNA complexity or ...