When the CRISPR Cas9 protein is added to a cell along with a piece of guide RNA, the Cas9 protein hooks up with the guide RNA and then moves along the strands of DNA until it finds and binds to a ...

Both Cas9 and Cas12a cleave DNA, but they differ in how they cut the molecule.

可识别并切割非完全配对的双链DNA、十字形结构DNA、 Holliday交叉DNA、异源双链DNA，或缓慢地切割带有切刻的双链DNA；可有效识别大于1个碱基的基因错配，广泛应用于由CRISPR-Cas9、锌指核酸酶(ZFNs ...

With vasa-Cas9, high efficiency and self-limiting suppression was achieved. Applying the CRISPR-Cas9 system to edit high GC content genomes has been challenging due to its high off-target activity.

这组作者使用基因编辑工具CRISPR-Cas9，让NANOS2基因失去活性 ... 这组作者使用精原干细胞移植（SSCT）实现了睾丸结构正常的小鼠、猪和山羊产生 ...

实验结果显示，target DNA确实可以在体外激活Cas12a的RuvC结构域来加工pre-crRNA的spacer区域。此外，团队成员也在其他V型CRISPR-Cas系统成员，如Cas12b、Cas12i和Cas12j，以及II型CRISPR-Cas9中观察到了这一现象。

Get Instant Summarized Text (Gist) CRISPR nucleases, Cas9 and Cas12, have been engineered to evade immune detection, addressing a key challenge in CRISPR-based therapies. By identifying and ...

CRISPR Therapeutics AG is a gene editing company, which engages in the development of transformative gene-based medicines for serious diseases using its proprietary CRISPR/Cas9 platform.

It's clear though that these companies need time. Even with the only approved CAS9 based treatment out there, CRISPR is still years away from achieving its potential. In a recent update to ...