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The IHC protocol was as follows: (a) deparaffinization of xylene and rehydration of gradient ethanol; (b) microwave-mediated thermal repair for 10 min in pH 6.0 citric acid (BL604A, Biosharp, China); ...

Briefly, the liver sections were dewaxed in xylene, rehydrated, and heated in a microwave with citrate buffer (pH 6.0) for antigen retrieval before exposure to the primary antibody. Then, sections ...

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