The Flow Cytometry and Cell Separation Facility provides advanced cell sorting and cell analysis techniques including immunofluorescence detection of 18 colors, cell-cycle distribution, apoptosis ...
Laser scanning cytometry (LSC) is the technology of choice ... make it a preferred choice for studies requiring simple or multiplexed cell cycle analysis of adherent cells. DNA damage studies ...
Harvest cells, want good single cell suspension Count cells Dispense aliquots of 1 million cells into labeled tubes Add 5 ml PBS and centrifuge for 5 min at 400 x g. Re-suspend cell pellet in 300 ul ...
After new users become familiar with flow cytometry instrumentation and learn how to set up the instrument and acquire data, the next topic to master is panel design. Optimizing a multicolor ...
After new users become familiar with flow cytometry instrumentation and learn how to set up the instrument and acquire data, the next topic to master is antibody panel design. Optimizing a multicolor ...