2022年8月7日 · GAPDH,Tubulin,Actin是WB实验中最常用的三种 上样内参 （loading control），因被认为表达水平受环境因素影响较小，在个体各个生长阶段的大多数或几乎全部组织中持续表达或变化很小，所以被称为 管家基因 （house-keeping genes），是WB实验中校正上样量、检测上样过程中 ...

2018年10月15日 · This paper reveals the intricate bidirectional actin–microtubule crosstalk necessary to establish polarization of microtubules and actin microvilli along the apico-basal axis of epithelial...

Tubulin and actin (the subunits of microtubules and actin filaments, respectively) bind and hydrolyze nucleoside triphosphates (tubulin binds GTP and actin binds ATP). Nucleotide hydrolysis underlies the characteristic dynamic behavior of these two filaments.

Microtubules are the largest type of filament, with a diameter of about 25 nanometers (nm), and they are composed of a protein called tubulin. Actin filaments are the smallest type, with a...

The presence of actin, tubulin, and intermediate filament homologs in these relatively simple cells suggests that primitive cytoskeletons first arose in bacteria. In bacteria such as Escherichia coli, homologs of tubulin and actin directly interact with each other and are crucial for coordinating cell growth and division. The function and ...

2021年8月18日 · Microtubule dynamics and their control are essential for the normal function and division of all eukaryotic cells. This plethora of functions is, in large part, supported by dynamic...

This review will discuss the interplay between heterotrimeric G protein signaling and elements of the cytoskeleton. Also described and discussed will be the interplay between tubulin and G proteins that results in atypical modulation of signaling pathways and cytoskeletal dynamics.

Tau protein association with tubulin immobilized in a solid phase support system was inhibited by actin monomer, and a higher inhibition was attained in the presence of preassembled actin filaments. Conversely, tubulin and assembled microtubules strongly inhibited tau interaction with actin in the solid phase system.

2021年11月25日 · Here we review basic structural and biochemical properties of actin and tubulin, and models for their polymerization dynamics that are rooted in chemical kinetics theory. We then review evidence for the existence of structural plasticity in these cytoskeletal polymers and discuss implications for their dynamics inside cells.

2021年1月28日 · Actin filaments and microtubules are essential cytoskeletal proteins that act together to endow cells with a foundation for shape, infrastructure for transport, and mechanical forces that drive and direct locomotion and cell division.