Cell markers for fibroblast characterization and analysis are used in several research applications including immunohistochemistry (IHC), immunocytochemistry (ICC) /immunofluorescence (IF), flow cytometry, and western blot. Additionally, RNAscope ® is a powerful tool allowing visualization of fibroblast marker gene expression in situ.

2020年7月31日 · Fibroblasts were identified by their archetypal markers LUM, DCN, VIM, PDGFRA, and COL1A2 27, constituted the most abundant skin cell type (5948 cells in total), and were represented by four clusters (#1, #2, #3, and #9, Fig. 1c and Supplementary Fig. 1c).

2024年12月30日 · The table below lists protein markers of fibroblasts mentioned by recent literature for characterizing distinct subpopulations. Marker information is accompanied by links to relevant antibody and ELISA kit products, as these are commonly used in marker immunodetection.

我们做肿瘤研究的单细胞数据，一般来说会选择初步很粗狂的定义大的细胞亚群，比如我常用的 第一次分群是通用规则是： immune (CD45+,PTPRC), epithelial/cancer (EpCAM+,EPCAM), stromal (CD10+,MME,fibo or CD31+…

2020年8月7日 · We reveal gene expression signatures that demarcate fibroblasts from mural cells and provide molecular signatures for cell subtype identification. We observe striking inter- and intra-organ...

2022年6月14日 · Fibroblasts, the principal cell type of connective tissue, secrete extracellular matrix components during tissue development, homeostasis, repair and disease. Despite this crucial role, the...

2021年6月17日 · In this study, we explore fibroblast heterogeneity in keloid, a paradigm of fibrotic skin diseases, by using single-cell RNA-seq. Our results indicate that keloid fibroblasts can be divided...

2023年5月4日 · In this review, we incorporate some new CAF markers including Meflin and CD200, and also delve into new mechanisms of the so-called “old” CAF markers acting on tumor cells, such as leading to immunosuppressive TME status.

2020年11月11日 · Cancer-associated fibroblasts (CAFs) are the key component of tumor stromal. High heterogeneity of CAFs reflects in their origin, phenotype and function. Biological function which can be suggested by biomarkers of distinct CAF subgroups may be different, even opposite, just like water and fire.

1995年7月7日 · We performed subtractive and differential hybridization for transcript comparison between murine fibroblasts and isogenic epithelium, and observed only a few novel intracellular genes which were relatively specific for fibroblasts. One such gene encodes a filament-associated, calcium-binding protein, fibroblast-specific protein 1 (FSP1).