2023年9月7日 · We show here that downregulation of Pallidin and other members of BLOC1 in the surface glia, the Drosophila equivalent of the blood-brain barrier, reduces and delays nighttime sleep in a circadian-clock-dependent manner.

2024年2月15日 · We show here that Drosophila glia exhibit a daily cycle of glial mitochondrial oxidation and lipid accumulation that is dependent on prior wake and requires the Drosophila APOE orthologs NLaz...

2020年3月23日 · To determine whether sleep is sufficient to promote Wallerian degeneration, we pharmacologically induced sleep in axotomized flies and measured neurite clearance. In Drosophila, the GABA A receptor agonist gaboxadol potently induces sleep (Figures S2A–S2C) . Flies expressing cd8:GFP in Or22a neurons were placed on gaboxadol-laced food ...

2025年1月3日 · To address a possible biological function of TrissinR expression in DN3s, we exploited our recent Drosophila GPCR gRNA library and recently characterized split GAL4 driver lines to knock out TrissinR only in DN3s .

Sleep is thought to be restorative to brain energy homeostasis, but it is not clear how this is achieved. We show here that Drosophila glia exhibit a daily cycle of glial mitochondrial oxidation and lipid accumulation that is dependent on prior wake and requires the Drosophila APOE orthologs NLaz an …

This study using Drosophila shows that oscillators in specific glia types maintain awakeness during the day by regulating the daily plasticity of clock neurons. Keywords: circadian clock, insect behavior, neuronal plasticity, sleep

2020年3月23日 · The fruit fly Drosophila melanogaster provides a powerful model for investigating the relationship between sleep and Wallerian degeneration [3]. Several lines of evidence suggest that glia influence sleep duration, sleep-mediated neuronal homeostasis, and clearance of toxic substances during sleep, raising the possibility that glial engulfment ...

2021年1月11日 · Powerful genetic tools in the fruit fly Drosophila melanogaster have identified — at an unprecedented level of detail — genes and neural circuits that regulate sleep. This research has revealed that the functions and neural principles of sleep regulation are largely conserved from flies to mammals.

Glia of the Drosophila blood/hemolymph-brain barrier (BBB) emerged as a new cellular locus of sleep regulation in this study, such that genetically manipulating endocytosis in these cells alone was sufficient to increase sleep. As the increased sleep appeared to reflect higher sleep need, we asked if sleep enhances endocytosis through the BBB ...

2023年9月19日 · Here, we find that ensheathing glia of Drosophila show the dynamics expected of a sleep homeostat. Calcium levels in these cells – monitored in central brain areas such as the central complex and mushroom bodies – integrate different kinds of behavioral effort across time and reset during sleep.