Mast cells (MCs) are tissue-resident immune cells that exhibit homeostatic and neuron-associated functions. Here, we combined whole-tissue imaging and single-cell RNA sequencing datasets to generate a pan-organ analysis of MCs in mice and humans at steady state.

2024年12月20日 · Mast cells, which constitute tissue-resident immune cells, are distributed in the dural meninges. Here, we provide procedural guidelines for investigating mouse dural mast cells using two techniques. First, we outline the procedures for dural tissue dissection, single-cell isolation, and subsequent surface staining for mast cell identification ...

These findings shed light on the functions of IEMCs, uncover a previously unrecognized phase of type 2 immunity involving mast cell-epithelial cell crosstalk, and advance our understanding of the cellular mechanisms underlying gasdermin C activation.

6 天之前 · ABSTRACT Background IgE-mediated food allergy is accompanied by mucosal mast cell (MMC) hyperplasia in the intestinal mucosa. ... Graphical Abstract. ... IL, interleukin; iMMC, induced mucosal mast cell; MC, mast cell; MHC-II, major histocompatibility complex class II, MMC, mucosal mast cell. Conflicts of Interest. The authors declare no ...

2024年11月13日 · • Both mouse connective tissue mast cells and human mast cells can contribute to IgG-mediated anaphylaxis by producing histamine in response to IgG/Ag – FcγR interactions. • IgG-responsive murine mast cells are a subset of IgE-responsive mast cells.

2021年7月7日 · Graphical Abstract The passive mast cell activation test (MAT), which uses mast cells (MCs) that were passively sensitized with patients' sera, is a promising diagnostic for IgE-mediated allergies. For autoimmune urticaria, in the autoimmune MAT, MCs can directly be activated with patients' sera or after passive sensitization with donor IgE.

To gain a better understanding of intestinal mast cells and their function, we examined the spatial distribution and dynamic alterations of intestinal mast cell populations during a typical type 2 immune response, modeled by infection with a widely used natural murine intestinal parasite Heligmosomoides polygyrus (H. polygyrus) (Figure 1A).

2021年4月15日 · Cutaneous mast cells mediate numerous skin inflammatory processes and have anatomical and functional associations with sensory afferent neurons. We reveal that epidermal nerve endings from a subset of sensory nonpeptidergic neurons expressing MrgprD are reduced by the absence of Langerhans cells.

2024年5月7日 · Mast cell activation was linked to a potential direct effect of the spike protein, the major surface protein of SARS-CoV-2. A mast cell deficient mouse model displayed markedly reduced lung pathology during infection, arguing for a direct contribution of mast cells to SARS-CoV-2 induced lung pathology in mice.

2015年12月1日 · Zhou et al. show that mast cells in white adipose tissues from lean humans and mice are leptin deficient and polarize macrophages toward anti-inflammatory M2 phenotype. Adoptive transfer of leptin-deficient mast cells mitigates obesity and diabetes in obese mice.