2022年6月1日 · We thus developed a cell atlas in which human and mouse trigeminal ganglia are transcriptionally and epigenomically profiled at single-cell resolution. These data describe evolutionarily conserved and human-specific gene expression patterns within each trigeminal ganglion cell type, as well as the transcription factors and gene regulatory ...

To circumvent the limitation, overlying brain tissue must to be removed to expose the TG, so that optical recording can access deep brain neural ensembles. This set of protocols describes a procedure for conducting non-survival surgery to visualize the TG in mice while mouse is alive.

Purpose : The trigeminal ganglion (TG) plays a pivotal role in eye diseases like neurotrophic and herpes keratitis. Despite the development of multiple mouse models to explore TG physiology and pathology, the understanding of mouse TG anatomy remains unclear, which has impacted interpretations of many datasets.

2022年6月1日 · • A human and mouse trigeminal ganglion (TG) cell atlas • Conservation of cell-type-specific gene expression between human and mouse TG • Epigenomic maps of TG offer insight into migraine-associated genetic variants • Neuronal and non-neuronal cell types are engaged in two mouse headache models

2022年6月2日 · Orofacial inflammation leads to transcriptional alterations in trigeminal ganglion (TG) neurons. However, diverse alterations and regulatory mechanisms following orofacial inflammatory pain in different types of TG neurons remain unclear. Here, orofacial inflammation was induced by injection of comp …

2022年6月1日 · We thus developed a cell atlas in which human and mouse trigeminal ganglia are transcriptionally and epigenomically profiled at single-cell resolution. These data describe evolutionarily conserved and human-specific gene expression patterns within each trigeminal ganglion cell type, as well as the transcription factors and gene regulatory ...

While single-cell transcriptomics has defined 15 different cell types in the mouse TG, the spatial organization of cells is lost due to the dissociation of tissue that is required prior to profiling. This study seeks to establish the first single-cell spatial atlas of the mouse trigeminal ganglion using spatial transcriptome technology.

2024年6月21日 · Harmonizing somatosensory sc/snRNA-seq datasets from multiple species enables direct cell-type–specific transcriptomic comparisons across species, which is particularly important for understanding similarities between human and mouse sensory ganglia, as mice are often used to interrogate cell-type function and model pathology.

2024年9月24日 · We studied terminal morphologies and tactile responses of lingual afferents from the trigeminal ganglion. Fungiform papillae, the taste-bud-holding structures in the tongue, were convergently innervated by multiple Piezo2 + trigeminal afferents, whereas single trigeminal afferents branched into multiple adjacent filiform papillae.

2010年2月24日 · Positional patterning of trigeminal ganglion primary sensory neurons sets the stage for the somatotopy of ophthalmic, maxillary and mandibular central afferents, transforming the spatial...