2010年11月21日 · Biochemical and genetic characterization of enzymes showed that PPA-AT directs carbon flux from prephenate toward arogenate, making the arogenate pathway predominant in plant phenylalanine...

2014年2月7日 · We present here the first identification of prephenate aminotransferases (PATs) in seven arogenate-competent microorganisms and the discovery that PAT activity is provided by three different classes of aminotransferase, which belong to two different fold types of pyridoxal phosphate enzymes: an aspartate aminotransferase subgroup 1β in tested ...

Here we identified Petunia hybrida and Arabidopsis thaliana genes encoding prephenate aminotransferases (PPA-ATs), thus completing the identification of the genes involved in phenylalanine and tyrosine biosyntheses.

In enzymology, glutamate-prephenate aminotransferase (EC 2.6.1.79, also known as prephenate transaminase, PAT, and L-glutamate:prephenate aminotransferase) is an enzyme that catalyzes the chemical reaction. Thus, the two substrates of this enzyme are L-arogenate and 2-oxoglutarate, whereas its two products are prephenate and L-glutamate.

1987年1月1日 · Prephenate aminotransferase from higher plants shows a marked preference for prephenate as substrate compared to phenylpy- ruvate or 4-hydroxyphenylpyruvate. The ability of at least some plant prephenate aminotransferases to withstand high temperatures up to 70 ° that inactivate other aminotransferase enzymes ~ is a striking and exploit- able ...

The aromatic amino acids L-phenylalanine and L-tyrosine and their plant-derived natural products are essential in human and plant metabolism and physiology. Here we identified Petunia hybrida and Arabidopsis thaliana genes encoding prephenate aminotransferases (PPA-ATs), thus completing the identifi …

Through phylogenetic, bioinformatic, and biochemical analyses of prephenate aminotransferase enzymes from deep taxonomic lineages, this study revealed unique evolutionary history and molecular changes of key enzymes responsible for phenylalanine biosynthesis in plants.

2017年7月28日 · Plants make tyrosine and phenylalanine by a different pathway from many microbes; this pathway requires prephenate aminotransferase (PAT) as the key enzyme. Prephenate aminotransferase produces arogenate, the unique and immediate precursor for both tyrosine and phenylalanine in plants, and also has aspartate aminotransferase (AAT) activity.

2018年2月5日 · Prephenate aminotransferase (PAT) catalyzes the pyridoxal 5′-phosphate-dependent transfer of an amine from an amino acid (aspartate) to a keto acid substrate (prephenate), resulting in a new amino acid (arogenate) and a new keto acid (oxaloacetate, OAA).

We present here the first identifica-tion of prephenate aminotransferases (PATs) in seven aroge-nate-competent microorganisms and the discovery that PAT activity is provided by three different classes of aminotrans-ferase, which belong to two different fold types of pyridoxal phosphate enzymes: an aspartate aminotransferase subgroup.