Structural and functional characterizations of mung bean …
Isolated mung bean mitochondrial nucleoids were visualized by thin-section electron microscopy to have an electron-dense chromatin-like structure associated with a vesicle component ; most of the chromatin-like elements appeared to be linked to or located immediately next to the vesicles.
Figure 1. Structure of mitochondria from plants and animals. (A) Electron micrograph of a mitochondrion in a mesophyll cell of broad bean (Vicia faba). (B) Time-lapse pictures showing a dividing mitochondrion in an Arabidopsis epidermal cell (arrowheads). All the visible organelles are mitochondria labeled with green fluorescent protein.
Correlation between mtDNA complexity and mtDNA replication …
2016年9月9日 · This study aimed to investigate the correlation between RDR and mtDNA complexity during mitochondrial development in mung bean (Vigna radiata) seed, and the initiation and processing of RDR in plant mitochondria. Flow cytometry, pulsed-field gel electrophoresis, electron microscopy, real-time PCR and …
Characterization of the Structure and DNA Complexity of Mung Bean …
2011年3月1日 · Electron microscopic images of mitochondrial nucleoids isolated from mung bean seedlings revealed a relatively homogeneous population of particles, each consisting of a chromatin-like structure associated with a membrane component.
Plant mitochondria. Fifty years of plant biology - PubMed
The invention of the electron microscope and the development of a technique of differential fractionation permitting the separation of the cell constituents, made it possible to connect the structure and function of mitochondria and to attribute …
Atomic structure of a mitochondrial complex I intermediate from ...
Here, we present the cryoEM structure of the known CI assembly intermediate CI* from Vigna radiata at 3.9 Å resolution. CI* contains CI’s NADH-binding and CoQ-binding modules, the proximal-pumping module and the plant-specific γ-carbonic-anhydrase domain (γCA).
1.7 Mitochondria – Plant Anatomy and Physiology
Mitochondria are the major site of ATP and energy production in plants and animals. Numbers vary from 20 to 100,000 per cell and they vary in form and activity. Mitochondria are double-membraned organelles (like the nucleus) so are said to be surrounded by an envelope.
Plantorganelle Hunter is an effective deep-learning-based method …
2023年9月25日 · Accurate delineation of plant cell organelles from electron microscope images is essential for understanding subcellular behaviour and function. Here we develop a deep-learning pipeline,...
For their investigation, Maldonado et al. iso-lated mitochondria from etiolated mung bean seedlings; the protein complexes of the electron transport chain were then purified, and their structure was analyzed using a new experimental strategy based on single-particle cryo-electron microscopy combined with computer-based image processing (Kuhlbran...
(PDF) Plant Mitochondria - ResearchGate
2020年10月29日 · Plant mitochondria have alternative ETC pathways to regulate mitochondrial ROS (mtROS) through modulating the rate of redox reactions, proton gradient and ATP production.